Erythropoietin gene-modified conditioned medium of human mesenchymal cells promotes hematopoietic development from human embryonic stem cells.
- Author:
Chao YANG
1
;
Lei JI
;
Wen YUE
;
Ruo-Yong WANG
;
Yan-Hua LI
;
Jia-Fei XI
;
Xiao-Yan XIE
;
Li-Juan HE
;
Xue NAN
;
Xue-Tao PEI
Author Information
1. Laboratory of Stem Cell and Regenerative Medicine, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
Cell Differentiation;
drug effects;
Culture Media, Conditioned;
pharmacology;
Embryonic Stem Cells;
cytology;
drug effects;
Erythropoietin;
genetics;
pharmacology;
Hematopoietic System;
Humans;
Mesenchymal Stromal Cells;
cytology;
metabolism;
Organisms, Genetically Modified
- From:
Journal of Experimental Hematology
2010;18(4):976-980
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells.
