Effects of riboflavin combined with photosensitization on reduction of Gram-positive and Gram-negative indicating germs in plasma and P-selectin expression of apheresis platelet concentrates.
- Author:
Xue-Yin ZHOU
1
;
Wen XIONG
;
Ling-Kui KONG
Author Information
1. Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
metabolism;
Drug Carriers;
Gram-Negative Bacteria;
drug effects;
radiation effects;
Gram-Positive Bacteria;
drug effects;
radiation effects;
Humans;
P-Selectin;
blood;
Photosensitizing Agents;
pharmacology;
Platelet Count;
Plateletpheresis;
methods;
Riboflavin;
pharmacology;
Ultraviolet Rays
- From:
Journal of Experimental Hematology
2010;18(4):1059-1062
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to confirm the practical efficacy of reducing indicating germs suspended in plasma by riboflavin and photosensitized inactivation and to evaluate its influence on activation of apheresis platelet concentrates. The synergistic effects of riboflavin combined with ultraviolet irradiation on inactivation of germs were investigated by using Escherichia Coli (E. coli) and Staphylococcus Aureus (S. aureus) as Gram⁻ and Gram(+) indicating germs, respectively. The activation status of apheresis-platelet concentrates treated with riboflavin combined with ultraviolet irradiation was detected by flow cytometry. The results showed that when 50 μmol/L of riboflavin was combined with 6.2 J/ml of ultraviolet irradiation, the T/E ratios reached 1.42 for E. coli and 1.68 for S. Aureus, and reduction of E. Coli and S. Aureus were 3.87 Logs and 3.82 Logs respectively; the CD62p expression level on germ-inactivated platelets stored at 22 degrees C for 0 and 5 days were 4.92% and 36.18% respectively, which slightly increased as compared with controls (3.94% and 32.03)% (p < 0.05). It is concluded that combination of riboflavin with ultraviolet irradiation displays well synergistic effects which can reduce E. Coli and S. Aureus counts, but no significantly influence on platelets. The partial activation of liquid platelets mainly presents metabolism damage during storage, which is found at an acceptable level.
