Cytogenetics of chronic lymphocytic leukemia stimulated by CpG-oligodeoxynucleotides and IL-2.
- Author:
Dong-Mei WANG
1
;
Wei XU
;
Hua-Jie DONG
;
Cheng FANG
;
Dan-Xia ZHU
;
Xin CAO
;
Hua-Yuan ZHU
;
Yun ZHUANG
;
Hai-Rong QIU
;
Hui YANG
;
Jian-Yong LI
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Provincial Hospital, Nanjing 210029, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Cell Line, Tumor;
drug effects;
Chromosome Aberrations;
Chromosome Banding;
Chromosome Disorders;
genetics;
Cytogenetics;
Female;
Humans;
Interleukin-2;
pharmacology;
Leukemia, Lymphocytic, Chronic, B-Cell;
genetics;
pathology;
Male;
Middle Aged;
Oligodeoxyribonucleotides;
pharmacology;
Translocation, Genetic
- From:
Journal of Experimental Hematology
2010;18(5):1114-1118
- CountryChina
- Language:Chinese
-
Abstract:
This study was to explore the stimulating effect of CpG-oligodeoxynucleotides (CpG-ODN) in combination with interleukin-2 (IL-2) on cytogenetic features of chronic lymphocytic leukemia (CLL) cells. Peripheral blood or bone marrow cells of 115 patients with CLL were cultured for 72 hours with CpG-ODN plus interleukin-2 (IL-2), and routine karyotype analysis was performed with R-banding technique. The metaphase number≥20 was considered as successful stimulation. The results showed that among the 115 CLL patients, successful stimulation rate was 74.8%. The rate of chromosome aberrations was 58.1%. One kind of aberration was detected in 21 cases (24.4%), two kinds of aberration in 6 cases (7.0%), complex aberrant karyotype in 23 cases (26.7%), included highly complex aberrant karyotype in 9 cases (10.5%), respectively. A total of 163 abnormalities of 102 kinds were detected in 86 patients. Number aberrations were 116 (71.2%), and structural abnormalities were 47 (28.8%). The most frequent number aberration was trisomy 12 (14.0%), and structural aberration was 15q+ (5.8%). It is concluded that most of CLL patients have chromosome abnormality, and the number abnormality are more frequent than the structural aberrations. CpG-ODN plus IL-2 can effectively raise the number of cells at metaphase and the detection rate of chromosome aberrations in CLL patients.
