Application of multiplex rt-PCR assay for screening rare or cryptic chromosome translocations in de novo patients with acute myeloid leukemia.
- Author:
Hai-Min CHEN
1
;
Hai-Yang YUAN
;
Xing FAN
;
Hai-Yan HE
;
Bing CHEN
;
Jing-Yi SHI
;
Yong-Mei ZHU
Author Information
1. Shanghai Institute of Hematology, Shanghai Ruijin Hospital, Shanghai Jiaotong University Medical College, Shanghai 200025, China.
- Publication Type:Journal Article
- MeSH:
Chromosome Banding;
Gene Rearrangement;
Genetic Testing;
Humans;
Leukemia, Myeloid, Acute;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Translocation, Genetic
- From:
Journal of Experimental Hematology
2010;18(5):1138-1142
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the clinical feasibility of using multiplex PT-PCR assay for screening rare/cryptic chromosome translocations in patients with de novo acute myeloid leukemia. For 126 patients with de novo AML-M4/M5 without common chromosome translocations including t(15;17), t(8;21) and t(16;16), 3 parallel multiplex RT-PCR assays were set up to detect 6 mll-related gene rearrangements (mll/af10, mll/af17, mll/ell, mll/af9, mll/af6 and mll/enl) with low detection rate and 4 rare fusion genes (dek/can, tls/erg, aml1/mds (evi1) and npm/mlf1). The results showed that 11 patients with positive result from 126 patients were detected which involved in 5 molecular abnormalities. Among them, 10 cases were AML-M5 (16.67%), 1 cases AML-M4 (1.51%). The marker chromosomes were observed in 2 cases out of 11 cases through conventional karyotyping analysis, the karyotyping analysis in 1 case was not performed because this case had 1 mitotic figure only, no any cytogenetic aberrations were found in other 8 cases through R-band karyotyping analysis. It is concluded that multiplex RT-PCR designed in this study can quickly, effectively and accurately identify the rare/cryptic chromosome translocations and can be used in clinical detection.
