Expression of Maf-b mRNA in de novo leukemia patients and its clinical significance.
- Author:
Li-Ping FAN
1
;
Jian-Zhen SHEN
Author Information
1. Department of Blood Transfusion, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Case-Control Studies;
Cell Proliferation;
Child;
Female;
Humans;
Leukemia;
genetics;
pathology;
Leukemia, Myeloid, Acute;
genetics;
pathology;
MafB Transcription Factor;
genetics;
Male;
Middle Aged;
RNA, Messenger;
genetics;
Young Adult
- From:
Journal of Experimental Hematology
2010;18(5):1147-1150
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to quantitatively detect the level of maf-b mRNA in leukemia patients and evaluate its clinical significance. Real-time fluorescence quantitative PCR was used to detect the relative expression level of maf-b mRNA. The expression change of maf-b mRNA in various types of leukemia was analyzed. Then, the relationship of maf-b mRNA expression with laboratory index and the response to chemotherapy was analyzed. The results showed that maf-b mRNA expression level in acute myeloid leukemia (AML) patients was lower than that in normal group (p<0.01) and positively correlated with white blood cell count (p<0.01) and the expression of CD34 (p<0.01). There was no correlation between maf-b mRNA expression level and chemotherapy response in AML patients except for acute promyelocytic leukemia (APL). Maf-b mRNA expression levels in acute lymphoid leukemia (ALL) and chronic myeloid leukemia (CML) patients were also lower than that in normal group (p<0.01). It is concluded that there is low expression of maf-b gene in AML patients. Abnormal expression of maf-b correlates with abnormal proliferation of AML cells, which may be a new prognostic factor for AML.
