Combined effect of anti-vascular endothelial growth factor antibody and recombinant human TRAIL on inducing apoptosis of leukemia K562 cells.
- Author:
A-Hui WANG
1
;
Zhi-Min ZHONG
;
Xiao-Mian ZHOU
Author Information
1. Department of Laboratory Medicine, Guangzhou First Municipal People Hospital, Affiliated to Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
pharmacology;
Apoptosis;
drug effects;
Humans;
K562 Cells;
TNF-Related Apoptosis-Inducing Ligand;
pharmacology;
Vascular Endothelial Growth Factor A;
immunology
- From:
Journal of Experimental Hematology
2010;18(5):1163-1167
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the synergistic effect of soluble human recombinant tumor necrosis factor related apoptosis inducing ligand (TRAIL) protein combined with anti-vascular endothelial growth factor (anti-VEGF) antibody on inducing apoptosis of leukemia K562 cells. The inhibitory rates and apoptotic rates of K562 cells treated with TRAIL and anti-VEGF antibody alone and their combination for 48 hours were examined by CCK-8 assay and flow cytometry respectively. The results indicated that the apoptotic rates of K562 cells induced with 75, 100 and 150 ng/ml TRAIL after culture for 48 hours were (4.26±0.67)%, (8.91±0.55)% and (11.71±0.78)% respectively. The apoptotic rates of K562 cells induced with 2.5, 5 and 7.5 µg/ml anti-VEGF antibody after culture for 48 hours were (3.95±0.69)%, (7.98±0.74)% and (10.26±0.83)% respectively. The apoptotic rates of K562 cells treated with combination use of 2.5 µg/ml anti-VEGF antibody and 75 ng/ml TRAIL, 5 µg/ml anti-VEGF antibody and 100 ng/ml TRAIL, and 7.5 µg/ml anti-VEGF antibody and 150 ng/ml TRAIL for 48 hours were (22.16±0.93)%, (36.32±1.31)% and (49.19±0.71)% respectively. The combined use of above mentioned agents induced significantly higher apoptosis and cytotoxicity than that of TRAIL or anti-VEGF antibody alone (p<0.05). It is concluded that the combination use of TRAIL and anti-VEGF antibody can significantly increase the sensitivity of K562 cells to apoptosis.
