Effects of xbp-1 gene silencing on bortezomib-induced apoptosis in human multiple myeloma cells.
- Author:
Yang YANG
1
;
Hong-Juan DONG
;
Guang-Xun GAO
;
Yi-Wei WANG
;
Hong-Tao GU
;
Mi-Mi SHU
;
Hua-Feng ZHU
;
Xie-Qun CHEN
Author Information
1. Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Boronic Acids;
pharmacology;
Bortezomib;
Cell Line, Tumor;
DNA-Binding Proteins;
genetics;
Gene Silencing;
Humans;
Multiple Myeloma;
genetics;
Pyrazines;
pharmacology;
RNA, Small Interfering;
genetics;
Regulatory Factor X Transcription Factors;
Transcription Factors;
genetics;
X-Box Binding Protein 1
- From:
Journal of Experimental Hematology
2010;18(5):1177-1180
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of xbp-1 gene silencing on bortezomib-induced apoptosis in multiple myeloma cell line NCI-H929 (H929). After xbp-1 gene expression was interfered by small hairpin RNA, the cell apoptosis was assayed by flow cytometry with Annexin V-FITC/PI staining, and the expression level of XBP-1 protein was detected by Western blot. The results showed that XBP-1 protein level of H929 cells was inhibited effectively by the PLL3.7 lentiviral vector mediated expression xbp-1 shRNA. The apoptosis rate was significantly higher in xbp-1 shRNA-expressing cells than in untreated control group [(10.13±0.61)% vs (2.5±0.2)%, p<0.05]. After treatment with bortezomib, the apoptosis rate of XBP-1 protein functionally deficient H929 cells was significantly higher than those in vector control group [(45.07±1)% vs (19.53±0.8)%, p<0.05]. It is concluded that xbp-1 gene silencing can significantly enhance the pro-apoptotic activity of bortezomib in multiple myeloma cells.
