Regulatory T cells inhibit proliferation of mouse lymphoma cell line EL4 in vitro.
- Author:
Chen ZHANG
1
;
Yan KONG
;
Jun GUO
;
Zhi-Tao YING
;
Zhi-Hong YUAN
;
Yun-Tao ZHANG
;
Wen ZHENG
;
Yu-Qin SONG
;
Ping-Ping LI
;
Jun ZHU
Author Information
1. Key Laboratory of Carcinogenesis and Translational Research Subodinated to Ministry of Education, Department of Lymphoma, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigen-Presenting Cells;
immunology;
Cell Line, Tumor;
Cell Proliferation;
Dendritic Cells;
immunology;
Female;
Forkhead Transcription Factors;
metabolism;
Interleukin-10;
secretion;
Interleukin-2 Receptor alpha Subunit;
immunology;
Lymphoma, T-Cell;
immunology;
pathology;
Mice;
Mice, Inbred C57BL;
T-Lymphocytes, Regulatory;
immunology;
Transforming Growth Factor beta1;
secretion
- From:
Journal of Experimental Hematology
2010;18(5):1198-1203
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of regulatory T (Treg) cells on the T cell lymphoma EL4 cells and its mechanism in vitro. C57BL/6 mouse Treg cells were isolated by magnetic cell sorting (MACS). The purity of Treg cells and their expression of Foxp3 were identified by flow cytometry (FCM) and PT-PCR respectively. The suppression of Treg cells on EL4 cells was detected by 3H-TdR method. At the same time, enzyme-linked immunosorbent assay (ELISA) was used to detect the secretion of cytokine TGF-β1 and IL-10. The results showed that CD4+CD25+ T cells could be successfully isolated by MACS with the purity reaching 94.52% and the expression of Foxp3 reaching 84.72%. After sorting, the expression of Foxp3 mRNA could be detected by RT-PCR. 3H-TdR assay confirmed that regulatory T cells could suppress the proliferation of EL4 cells with or without antigen presenting cells (APC) or dendritic cells (DC), APC or DC might effectively enhance the suppression. In addition, DC alone also suppressed the proliferation. TGF-β1 and IL-10 could be detected in the supernatant by ELISA. It is concluded that the Treg cells can obviously suppress the proliferation of T cell lymphoma cells in vitro, APC or DC can enhance this suppressive effect, while the DC alone also can suppress the proliferation of EL4 cells, the TGF-β1 and IL-10 cytokine pathway may be one of the mechanisms of suppression.
