Karyotype analysis using cells cultured from fetal bladder centesis.
- VernacularTitle:TCF7L2基因rs7903146C/T多态性与延边地区朝鲜族和汉族代谢综合征的相关性
- Author:
Jian GAO
1
;
Li-min RONG
;
Bing MEI
;
Xiao-ping YU
;
Wen-chao GUO
;
Yali LI
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Female; Humans; Karyotyping; Pregnancy; Prenatal Diagnosis; methods; Urinary Bladder; abnormalities
- From: Chinese Journal of Medical Genetics 2013;30(2):207-209
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of karyotype analysis using cells cultured from fetal bladder centesis samples.
METHODSSamples were derived from fetal bladder centesis for 3 fetuses featuring giant bladder and oligohydramnios. Following in vitro culture, cells were routinely processed and stained for chromosome analysis.
RESULTSFor all 3 cases, cell culture has achieved success. Sufficient metaphase cells were obtained for chromosome counting and karyotype analysis. The karyotypes of the 3 fetuses were respectively 46, XY, 46, XX, t(1;5)(q22;q12)[7]/46, XX[4], and 46, XY.
CONCLUSIONCells cultured from fetal bladder centesis may be used for karyotype analysis following in vitro culturing. This new approach can enable prenatal chromosome analysis for fetuses featuring smaller gestational weeks, giant bladder and oligohydramnios.
