Analysis of 89 amniotic samples using fluorescent in situ hybridization.
- VernacularTitle:ETS1基因与中国北方汉族人群系统性红斑狼疮的相关性研究
- Author:
Ping WEI
1
;
Yun-xing LI
;
Lan ZENG
;
Chun CHEN
;
Sheng-fang QIN
;
Xue-yan WANG
;
Na XI
;
Shu-qin TANG
;
Mei LENG
;
Chun-lan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Amniotic Fluid; cytology; Cell Culture Techniques; Female; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; Pregnancy
- From: Chinese Journal of Medical Genetics 2013;30(2):214-217
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the value of fluorescent in situ hybridization (FISH) for detecting common chromosome aneuploidies in interphase nuclei of amniotic fluid cells.
METHODSEighty two uncultured amniotic fluid samples and supernatants from 2 successfully and 5 unsuccessfully cultured amniotic fluid samples were analyzed with FISH. Results from standard cytogenetic analysis of 79 uncultured amniotic fluid samples and 2 successfully cultured amniotic fluid samples were compared with FISH results.
RESULTSAll of the 89 samples were succeeded analyzed with FISH. Positive findings included 3 cases with trisomy 21, 1 case with 47, XYY and 1 case with 69, XXX, which were consistent with results of karyotype analysis.
CONCLUSIONFISH is a rapid and accurate method for prenatal diagnosis, and can also provide a remedy to failed amniotic fluid cells culture.
