Frequencies of 9 bp deletion of mitochondrial DNA in ethnic Miao, Buyi and Dong from Guizhou.
- Author:
Yan HE
1
;
Ke-ren SHAN
;
Lin-yan REN
;
Ting ZHANG
;
Chan-juan WANG
;
Yuan XIE
;
Chan ZHANG
;
Lin-jie LI
;
Zhi-zhong GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; ethnology; DNA, Mitochondrial; genetics; Gene Deletion; Humans; Male; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism, Genetic; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2013;30(2):233-236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the frequency of a 9 bp deletion polymorphism of mitochondrial DNA (mtDNA) in ethnic Miao, Buyi and Dong populations from Guizhou province.
METHODSPolymerase chain reaction-polyacrylamide gel electrophoresis (PCR-PAGE) was used to detect the 9 bp deletion. The result was verified with DNA sequencing.
RESULTSTwo polymorphisms, including a standard pattern and a short pattern (the 9 bp deletion), were found among the three ethnic groups. The frequency of short pattern in 304 males was 23.0%. Respectively, those of Miao, Buyi and Dong ethnics were 28.6%, 26.8% and 13.7%. A statistically significant difference was detected among the three groups (P<0.05).
CONCLUSIONThe frequencies of the 9 bp polymorphism were relatively high among ethnic Miao, Buyi and Dong populations from Guizhou, and there was a significant difference between the three.
