Expression of FOS protein in glioma and its effect on the growth of human glioma cells.
- Author:
Tao TAO
1
;
Xiaoming LU
;
Lei YAO
;
Jiajia WANG
;
Yan SHI
;
Hui LUO
;
Ning LIU
;
Yongping YOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; genetics; Cell Proliferation; Cyclin D1; genetics; metabolism; Gene Expression; Glioma; genetics; metabolism; pathology; Humans; Matrix Metalloproteinase 9; genetics; metabolism; Neoplasm Grading; Proto-Oncogene Proteins c-fos; genetics; metabolism; RNA Interference
- From: Chinese Journal of Medical Genetics 2013;30(3):293-296
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of FOS protein in human glioma tissues and its effect on tumor growth.
METHODSFOS protein expression in glioma tissues was determined with immunohistochemical (IHC) staining. Subsequently, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression.
RESULTSThe expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells.
CONCLUSIONThe expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.
