Molecular basis and clinical blood transfusion for cases with ABO typing discrepancy.
- VernacularTitle:ABO血型正反定型不一致样本的分子机制及其临床输血分析
- Author:
Zhe ZHANG
1
;
Gang DENG
;
Dandan HUANG
;
Wenyu GUO
;
Deyi XU
;
Yong DU
;
Xiaoxian TANG
;
Yong YU
;
Danfei DENG
Author Information
- Publication Type:Journal Article
- MeSH: ABO Blood-Group System; genetics; Adult; Base Sequence; Blood Grouping and Crossmatching; Blood Transfusion; Exons; Genotype; Humans; Middle Aged; Young Adult
- From: Chinese Journal of Medical Genetics 2013;30(3):326-329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular genetic basis of samples with ABO typing discrepancy and provide the guidline for identification and clinical transfusion for these samples.
METHODSSix cases with similar serological characteristics were collected. Serological method, PCR-SSP and direct sequencing of ABO gene were used to explore the underlying mechanism. Condition of clinical transfusion of patients was also reviewed.
RESULTSThree conditions were related with the ABO blood type discrepancy, which included weaken antigen (2 cases), weakened antibody (3 cases) and ABO subtype (1 case). The satisfactory effect of transfusion was achieved in all patients with the principle of the same blood type or the compatible crossmatch.
CONCLUSIONHeterogeneity has existed with the ABO group. Indivianals with same reaction pattern may result in different mechanisms.
