Experimental study of NK4 gene targeted therapy mediated by human telomerase reverse transcriptase promoter for colon cancer.
- Author:
Bo XU
1
;
Huan-qing XIAO
;
Shan-qi ZENG
;
Gui-tian HUANG
;
Zhen-bang LIU
;
Yue-jun QIAN
;
Shu-hua LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; genetics; metabolism; pathology; therapy; Genetic Therapy; Humans; Mice; Mice, Nude; Neoplasm Invasiveness; Neoplasm Metastasis; Promoter Regions, Genetic; Telomerase; genetics; Transfection; Xenograft Model Antitumor Assays
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(11):851-854
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate anti-tumor effect of the recombined adenovirus encoding NK4 gene regulated by human telomerase reverse transcriptase (HTERT) promoter (Ad HTERTp-NK4) on human colon cancer.
METHODSColon cell line HCT116 was infected with Ad HTERTp-NK4. NK4 expression was determined by RT-PCR and Western blot. The cell-growth inhibition rate and the invasive capacity of cells were evaluated by MTT method and reconstituted basement membrane invasion assay. The model of subcutaneous tumor was generated by injection of HCT116 cells into the dorsum of nude mice. Ad HTERTp-NK4 was injected around the tumor tissues, and tumor growth was observed.
RESULTSNK4 gene was highly expressed in infected HCT116 cells. The cell growth inhibition rate and the invasive inhibition rate in Ad HTERTp-NK4 cells were 47.14% and 40.63% respectively, which were significantly higher than those in the control cells (2.75% and 2.31%, P<0.05). Tumor growth was significantly inhibited in mice injected with Ad HTERTp-NK4, and the tumor growth inhibition rate was 47.3%, which was significantly higher than that in the controls (4.6%, P<0.05).
CONCLUSIONAd HTERTp-NK4 can inhibit tumor growth and decrease the invasive capacity of tumor cells, which makes it an ideal candidate for new gene therapy for colon carcinoma.
