Effects of combined therapy of LY294002 and SN50 on nude mice model with gastric cancer.
- Author:
Jia-Lei SUN
1
;
Bao-Song ZHU
;
Wei GONG
;
Peng ZHANG
;
Li-Yan YU
;
Kui ZHAO
;
Chun-Gen XING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Chromones; pharmacology; Enzyme Inhibitors; pharmacology; Female; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Morpholines; pharmacology; NF-kappa B; antagonists & inhibitors; Peptides; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Stomach Neoplasms; metabolism; pathology; Tumor Suppressor Protein p53; metabolism; Xenograft Model Antitumor Assays; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2011;14(5):364-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of phosphatidylinositol 3-kinase inhibitor LY294002 combined with NF-κB P65 nuclear translocation inhibitor SN50 on the tumor cell growth and apoptosis using a nude mouse model of gastric cancer.
METHODSHuman gastric cancer cell strain SGC7901 was transplanted subcutaneously to nude mice to establish tumor models. Model mice were randomly divided into the control group, the LY294002 treatment group, the SN50 treatment group, and the LY294002+SN50 treatment group, with 5 in each group. After being treated for 10 days, the inhibition rate of tumor growth was ascertained by measuring the size of tumor. Immunohistochemical method was used to detect the expression levels of Bcl-2, P53 and Bax proteins and transmission electron microscopy to investigate the apoptosis of tumor cells.
RESULTSOn the 10th day after treatment, the inhibition rate of gastric cancer cellular growth in the LY294002+SN50 group was (49.2±2.5)%, which was significantly higher than that in the LY294002 group(29.4±1.5)% and SN50 group (19.7±1.6)%(P<0.05). In comparison with the other two groups, LY294002+SN50 group exhibited more severe apoptosis, with expression of Bcl-2 decreased and that of P53 and Bax increased more significantly(P<0.05).
CONCLUSIONLY294002 combined with SN50 inhibits the growth of SGC7901 transplanted tumor and aggravates the apoptosis of gastric cancer cells in nude mice model.
