Optimization of fermentation of recombinant human Endostatin (rh-Endostatin) expression in Escherichia coli.
- Author:
Guo-Dong CHANG
1
;
Zhuang-Lin LI
;
Jia-Yang QIN
;
Cui-Qing MA
;
Yong-Zhang LUO
;
Ping XU
Author Information
1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
- Publication Type:Journal Article
- MeSH:
Endostatins;
biosynthesis;
genetics;
Escherichia coli;
genetics;
growth & development;
metabolism;
Fermentation;
Humans;
Protein Engineering;
Recombinant Proteins;
biosynthesis;
genetics;
Thiogalactosides;
chemistry
- From:
Chinese Journal of Biotechnology
2005;21(4):662-666
- CountryChina
- Language:Chinese
-
Abstract:
The fermentation process of recombinant human Endostatin expression in Escherichia coli BL21 (DE3) was studied. The effects of factors such as concentration of IPTG, induction time, cultivation temperature and feeding strategies were investigated. Beside that, by changing the temperature to 40 degrees C after induction, the high-density cultivation finished in a much shorter period. After 9 hours cultivation, the optical density (OD) at 600 nm reached 140 and the yield of inclusion body was 3 g/L. While E. coli system was used, protein with better activity and stability was obtained. The cost was much lower and the producing process was much steadier. It will meet the demands of the industrial production.
