Enhancement of immune responses to hepatitis B DNA vaccine by superantigen SEA in mice.
- Author:
Yan-Wen JIN
1
;
Ping LI
;
Quan-Bin XU
;
Xuan LIU
;
Wei HUANG
;
Yun-Long WANG
;
Cheng CAO
;
Qing-Jun MA
Author Information
1. Beijing Institute of Biotechnology, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
Animals;
Enterotoxins;
immunology;
Hepatitis B;
immunology;
prevention & control;
therapy;
Hepatitis B Antibodies;
blood;
Hepatitis B Vaccines;
immunology;
Interferon-gamma;
secretion;
Mice;
Mice, Inbred BALB C;
Staphylococcus aureus;
immunology;
Superantigens;
immunology;
T-Lymphocytes, Cytotoxic;
immunology;
Vaccination;
Vaccines, DNA;
immunology
- From:
Chinese Journal of Biotechnology
2005;21(5):681-685
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the adjuvant effect of plasmid DNA encoding superantigen SEA (D227A) (pmSEA) on immune responses induced by HBV DNA vaccine containing HBV preS2 and S antigen in BABL/c (H-2d). BALB/c mice were immunized intramuscular injection with HBV DNA vaccine (pHBVS2S) mixed with or without pmSEA plasmid. Antibodies againat HBV PreS2 and S antigen in the sera were accessed by Anti-HBs ELISA, and the HBsAg specific cytotoxic T lymphocytes (CTLs) activity was determined by 5 Chromium Release Assay. The HBs peptide-specific IFN-gamma secreting T cells were detected by ELISPOT. Anti-HBs antibody titers and CTLs activity in mice immunized with pmSEA + pHBVS2S group were significant higher (P < 0.05) than pHBVS2S DNA vaccine group. The ratio of IgG1/IgG2a (0.282) was apparently different from the group immunized with peptide (10). Mice immunized with HBV DNA vaccine plus adjuvant produce higher titer of IgG1 and IgG2a antibodies against HBV S antigen 1.36 and 1.73 time higher than that without adjuvant respectively. HBs peptide--specific IFN-gamma secreting T cells increased 2 - 3 times by the pmSEA adjuvant, compared to DNA vaccine group. HBV DNA vaccine (pHBVS2S) induces humoral and cellular immuno-responses in BALB/c mice, and the responses could be significantly boasted by the plasmid encoding mSEA. Therefore the pmSEA was a potential adjuvant for DNA vaccines.
