The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism.
- Author:
Zhen-Min YE
1
;
Ye-Han ZHU
;
Wei-Hua SHENG
;
Jing-Cheng MIAO
;
Shao-Hua WEI
;
Kang WU
;
Ji-Cheng YANG
Author Information
1. Cell and Molecular Biology Institute, College of Medicine, SooChow University, Suzhou 215007, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Escherichia coli;
genetics;
metabolism;
Female;
HeLa Cells;
Humans;
Interleukins;
biosynthesis;
genetics;
immunology;
Mitochondria;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2005;21(5):719-724
- CountryChina
- Language:Chinese
-
Abstract:
The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell: inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6, TNF-alpha, IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
