The growth characteristics and ginsenosides isolation of suspension-cultured crown gall of Panax quinquefolium.
- Author:
Rong-Min YU
1
;
Qian-Xing JIN
;
Hui SUN
;
Wen-Cai YE
;
Yu ZHAO
Author Information
1. Department of Phytochemistry and Pharmacognosy, College of Pharmacy, Jinan University, Guangzhou 510632, China. rongminyu99@hotmail.com
- Publication Type:Journal Article
- MeSH:
Culture Media;
Culture Techniques;
methods;
Ginsenosides;
biosynthesis;
isolation & purification;
Panax;
growth & development;
metabolism;
Plants, Medicinal;
growth & development;
metabolism
- From:
Chinese Journal of Biotechnology
2005;21(5):754-758
- CountryChina
- Language:English
-
Abstract:
The growth characteristics and ginsenosides isolation of the suspension-cultured crown gall of Panax quinquefolium were studied. The result showed that the maximum biomass of cultures was 18.6 g/L (dry weight) and the content of ginsenosides reached its maximum level of 620.4 mg/L on the 27th day. The utilization rates of sugar, phosphorus, nitrogen in NH4+ and nitrogen in NO3- were 91.8%, 100%, 81% and 97%, respectively. Four compounds were isolated from the suspension-cultured crown gall and their structures were elucidated as pseudoginsenoside F11 (I), ginsenoside Rd (II), ginsenoside Rb1 (llI) and ginsenoside Rb3 (IV).
