Kinetics model of spherical immobilized cellulase.
- Author:
Jian-Qin ZHOU
1
;
Shi-Gong CHEN
;
Zhong-Kui ZHU
Author Information
1. School of Pharmacy, Soochow University, Suzhou 215007, China.
- Publication Type:Journal Article
- MeSH:
Cellulase;
metabolism;
Diffusion;
Enzymes, Immobilized;
metabolism;
Kinetics;
Microspheres;
Models, Chemical;
Particle Size;
Substrate Specificity
- From:
Chinese Journal of Biotechnology
2005;21(5):799-803
- CountryChina
- Language:Chinese
-
Abstract:
A kinetics model was developed for predicting and simulating immobilized cellulase performance, which follows Michaelis-Menten kinetics with competitive product inhibition. Taking into account the effects of competitive product inhibition, inner diffusional limitation, substrate concentration and carrier size, the substrate distribution and the product distribution in carriers were investigated, and the effectiveness factors were also calculated over a wide range of parameters. The effects of competitive product inhibition are shown to increase the substrate concentration in the carrier, and, additionally, to increase the effectiveness factors slightly. With the increase of inner diffusion coefficient, both the effectiveness factors and the substrate concentration in the carrier increase. As the carrier size increases, on the other hand, these values decrease. The effectiveness factors and the substrate concentration in the carrier are found to increase when substrate concentration in the reaction system increases.
