Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology.
- Author:
Xuan-Ming SHI
1
;
Yuan-Ming LUO
;
Gui-Feng ZHANG
;
Zhi-Guo SU
;
Yu-Bi HUANG
;
Ke-Qian YANG
Author Information
1. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
analysis;
Cell Membrane;
chemistry;
Genome, Bacterial;
genetics;
Mass Spectrometry;
methods;
Membrane Proteins;
analysis;
Proteome;
analysis;
genetics;
Streptomyces coelicolor;
chemistry;
genetics
- From:
Chinese Journal of Biotechnology
2005;21(5):814-819
- CountryChina
- Language:Chinese
-
Abstract:
Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
