The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV.
- Author:
Ya-Xiang XU
1
;
Xiao-Feng WU
;
Yu-Fang ZHU
;
Zi-Rong XU
;
Wei-De SHEN
Author Information
1. Suzhou University, Suzhou 215006, China. szdxxyx@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Baculoviridae;
genetics;
metabolism;
Bombyx;
genetics;
metabolism;
virology;
Genetic Vectors;
Penaeidae;
virology;
Recombinant Fusion Proteins;
genetics;
Viral Envelope Proteins;
genetics;
metabolism;
Virus Replication;
White spot syndrome virus 1;
genetics
- From:
Chinese Journal of Biotechnology
2005;21(5):837-839
- CountryChina
- Language:Chinese
-
Abstract:
Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus (WSSV). VP19 is a vesicle membrane protein of WSSV. HyNPV (Hybrid of AcNPV and BmNPV) constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vp19 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.
