Study on breeding up high-yield strain of taxol by protoplast mutagensis.
- Author:
Kai ZHAO
1
;
Dong-Po ZHOU
;
Wen-Xiang PING
;
Jun LIU
;
Xi MA
;
Tao JIN
Author Information
1. Life and Science College of Heilongjiang University, Harbin 150080, China.
- Publication Type:Journal Article
- MeSH:
Ascomycota;
drug effects;
genetics;
growth & development;
metabolism;
Fermentation;
Genetic Variation;
Mutagenesis;
Nystatin;
pharmacology;
Paclitaxel;
biosynthesis;
Protoplasts;
metabolism
- From:
Chinese Journal of Biotechnology
2005;21(5):848-851
- CountryChina
- Language:Chinese
-
Abstract:
In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it's ability to produce taxol was improved from 314.07 microg/L (strain NCEU-1) to 418.24 microg/L (strain U04-5).
