Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction and respiratory syndrome virus.
- Author:
Yun-Bo JIANG
1
;
Liu-Rong FANG
;
Shao-Bo XIAO
;
Hui ZHANG
;
Huan-Chun CHEN
Author Information
1. Laboratory of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Genetic Vectors;
Herpesvirus 1, Suid;
genetics;
immunology;
Mice;
Mice, Inbred BALB C;
Porcine respiratory and reproductive syndrome virus;
genetics;
immunology;
Recombinant Fusion Proteins;
genetics;
immunology;
Recombinant Proteins;
biosynthesis;
genetics;
immunology;
Swine;
Viral Envelope Proteins;
biosynthesis;
genetics;
Viral Vaccines;
genetics;
immunology
- From:
Chinese Journal of Biotechnology
2005;21(6):858-864
- CountryChina
- Language:Chinese
-
Abstract:
Pseudorabies virus (PRV), an alpha-herpesvirus, has been used as a vector for live-viral animal vaccines. The recombinant PRV TK- / gE- / GP5+, which expressing GP5 of PRRSV, is developed based on the PRV genetic-depleting vaccine-virus strain, TK- / gE- /LacZ+. However, this strain stimulated poorly the vaccinated animals to produce neutralizing antibodies against PRRSV. In order to develop a booster specific immunized response of the PRV recombinant, the ORF5 gene of PRRSV TK- / gE- / LacZ+ was substituted by a modified ORF5 gene, ORF5m. The resultant recombinant PRV, TK- /gE- / GP5m+, was verified by PCR, Southern blotting and Western blotting. TK- / gE- / GP5m+ and TK- / gE- / GP5+ expressed GP5 proteins were inoculated into balb/c mice to evaluate their immunogenicity. The results demonstrated that the amount of neutralization antibodies and cell-immunity responses induced by TK- / gE- /GP5m+ against PRRSV were higher than that of TK- / gE- / GP5+. This study indicated that the new recombinant PRV expressing the modified GP5m protein is a candidate for the development of bivalent genetic engineering vaccines against PRRSV and PRV.
