Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis).
- Author:
Hong-Xia QIN
1
;
Zhi-Ping JIA
;
Hai-Chao ZHANG
;
Jing-Mei LIU
;
Yu-Xia SONG
Author Information
1. College of Life Science, Ningxia University, Yinchuan 750021, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Cloning, Molecular;
Cold Temperature;
Droughts;
Expressed Sequence Tags;
Gene Expression Regulation, Plant;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
genetics;
metabolism;
Populus;
genetics;
metabolism;
Stress, Physiological;
Transcription Factors;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2005;21(6):906-910
- CountryChina
- Language:Chinese
-
Abstract:
Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.
