Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production.
- Author:
Ji-Na ZHANG
1
;
Xiu-Ping HE
;
Xue-Na GUO
;
Nan LIU
;
Bo-Run ZHANG
Author Information
1. The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology of Chinese Academy of Sciences, Beijing, 100080, China.
- Publication Type:Journal Article
- MeSH:
Acetolactate Synthase;
genetics;
metabolism;
Beer;
microbiology;
Cloning, Molecular;
Diacetyl;
metabolism;
Fermentation;
Gene Expression Regulation, Fungal;
Glutamate-Cysteine Ligase;
genetics;
metabolism;
Glutathione;
biosynthesis;
Metallothionein;
genetics;
metabolism;
Organisms, Genetically Modified;
genetics;
Saccharomyces cerevisiae;
genetics;
metabolism;
Saccharomyces cerevisiae Proteins;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2005;21(6):942-946
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected. The results of fermentation in 100-L bioreactor showed that the lagering time of beer produced for recombinant strain T2 was shortened by 3 days and the shelf life of the beer was prolonged about 50%. It may be more acceptable for the commercial application, as it does not contain foreign DNA.
