Study on rhG-CSF modified with polyethylene glycol.
- Author:
Lin-Lin ZHANG
1
;
Chun-Yang ZHENG
;
Jian-Du LEI
;
Guang-Hui MA
;
Zhi-Guo SU
;
Li WANG
Author Information
1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Granulocyte Colony-Stimulating Factor;
chemistry;
isolation & purification;
Humans;
Polyethylene Glycols;
chemistry;
Recombinant Proteins;
chemistry;
isolation & purification;
Surface Properties
- From:
Chinese Journal of Biotechnology
2005;21(6):965-969
- CountryChina
- Language:Chinese
-
Abstract:
Monomethoxy Polyethylene Glycol(mPEG20000) was activated by N-hydroxysuccinimede and analyzed by infrared spectrum and hydrolysis kinetics. In order to propose the optimized reaction conditions of mono-PEGylated rhG-CSF, orthogonal design of the experiment was investigated. Ion exchange chromatography was used to separate and purify PEGylated rhG-CSF from unPEGylated rhG-CSF. The purity of mono-PEGylated rhG-CSF was analyzed by high performance liquid chromatography (HPLC) to be 97%.
