Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis.
- Author:
Qiu-Li LI
1
;
Yi ZHANG
;
Hui YIN
;
Dan LI
Author Information
1. College of Life Science, Liaoning Normal University, Dalian 116029, China. liqiuli@dl.cn
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Betaine-Aldehyde Dehydrogenase;
genetics;
Chenopodiaceae;
enzymology;
genetics;
Cold Temperature;
Gene Expression Regulation, Enzymologic;
Gene Expression Regulation, Plant;
Genes, Plant;
Molecular Sequence Data;
Plant Proteins;
genetics;
Promoter Regions, Genetic;
Salts;
Sequence Analysis, DNA;
Transcription Initiation Site
- From:
Chinese Journal of Biotechnology
2006;22(1):77-81
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the 5' -flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements: salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.
