High-level expression of phenylalanine ammonia-lyase in Lactococcus Lactis via synthesized sequence based on bias codons.
- Author:
Xing CHEN
1
;
Bin GAO
;
Xing-Yuan JIA
;
Chang SU
;
Yue-Ping LÜ
;
Zhan-Yong WANG
;
Xin-Ping FAN
;
Bai XIAO
;
Jing-Zhong LIU
Author Information
1. Beijing Chaoyang Hospital Affiliate of Capital University of Medical Sciences, Beijing 100020, China.
- Publication Type:Journal Article
- MeSH:
Cloning, Molecular;
Codon;
genetics;
Genetic Vectors;
genetics;
Lactococcus lactis;
genetics;
metabolism;
Phenylalanine Ammonia-Lyase;
biosynthesis;
genetics;
Recombinant Proteins;
biosynthesis;
metabolism;
Transformation, Bacterial
- From:
Chinese Journal of Biotechnology
2006;22(2):187-190
- CountryChina
- Language:Chinese
-
Abstract:
To construct a safer and more efficient gene engineering Lactococcus Lactis for expressing phenylalaine ammonia lyase (PAL) which will be benefit for PKU therapy, pal cDNA of Parsly and synthesized sequence based on Lactococcus Lactis bias codons were recombined into two Lactococcus Lactis NICE systems. The activities of the expressed PAL were detected, and the effect of Lactococcus Lactis bias codons on the expression of exterior protein was analyzed. The results showed that the expression level of PAL was increased by using Lactococcus Lactis bias codons in both Lactococcus Lactis NICE systems. Through which several safer andmore efficient strains of the gene engineering Lactococcus Lactis were obtained.
