Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment.
- Author:
Tao XU
1
;
Bao-Cun ZHAO
;
Rong-Chao GE
;
Yin-Zhu SHEN
;
Zhan-Jing HUANG
Author Information
1. The College of Biology, Hebei Normal University, Shijiazhuang 050016, China.
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
Biolistics;
DNA, Plant;
genetics;
Glycogen Synthase Kinases;
genetics;
Mutation;
Plant Proteins;
genetics;
Plants, Genetically Modified;
Salt-Tolerant Plants;
genetics;
Seeds;
genetics;
Sodium Chloride;
metabolism;
Transformation, Genetic;
Triticum;
enzymology;
genetics;
physiology
- From:
Chinese Journal of Biotechnology
2006;22(2):211-214
- CountryChina
- Language:Chinese
-
Abstract:
The Tagsk1 (Triticum asetium L. glycogen synthase kinase 1) gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 (AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.
