Effects of siRNA targeted to survivin in suppressing proliferation and inducing apoptosis in breast cancer MCF-7 cells.
- Author:
Hai-tao GUAN
1
;
Xing-huan XUE
;
Xi-jing WANG
;
Ang LI
;
Zhao-yin QIN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Female; Genetic Therapy; Genetic Vectors; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; biosynthesis; genetics; physiology; Neoplasm Proteins; biosynthesis; genetics; physiology; RNA Interference; RNA, Messenger; biosynthesis; genetics; RNA, Small Interfering; genetics; metabolism; pharmacology; Transfection
- From: Chinese Journal of Oncology 2006;28(5):326-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBlocking the expression of survivin with RNA interference techniques, the effects of suppressing proliferation and inducing apoptosis of breast cancer MCF-7 cells were investigated.
METHODSA siRNA eukaryotic expression vector against survivin was constructed and transfected into breast cancer MCF-7 cells with lipofectamine 2000. The changes of survivin expression were detected by semi-quantitive RT-PCR and immunohistochemistry. The effect of suppressing proliferation of MCF-7 cell was detected by MTT assay. The effect of inducing MCF-7 cell apoptosis was detected by TUNEL assay.
RESULTSThe sequence-specific siRNA can efficiently block the expression of survivin both at mRNA and protein levels. The expression inhibition rate was 64.9% at mRNA level detected by semi-quantitive RT-PCR and 79.7% at protein level detected by immunohistochemistry. Blocking the expression of survivin can suppress proliferation of MCF-7 cells significantly. At 24 and 48 h after the cells were reseeded, the proliferation inhibition rates were 31.6% and 33.0%, respectively. At 24 h after transfection, apoptosis was induced in 12.9% of the cells as detected by TUNEL assay.
CONCLUSIONBlocking the expression of survivin with RNA interference technology can significantly suppress proliferation of MCF-7 cells and induce apoptosis to a certain degree. RNAi targeted to survivin has a potential value in gene therapy of breast cancer.
