Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.
- Author:
Feng WU
1
;
Qing-Ming WANG
;
Guo-Cai FAN
;
Ji-Zhong CHEN
;
Hui-Peng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; Female; Galectin 1; metabolism; Humans; Keratin-8; metabolism; Paclitaxel; pharmacology; Phosphopyruvate Hydratase; metabolism; Proteomics; methods; Ribosomal Proteins; metabolism
- From: Chinese Journal of Oncology 2006;28(6):418-421
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.
METHODSIn this study, the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis (2-DE), and compared with those from untreated MCF-7 cells. The differential proteins were identified by mass spectrometry.
RESULTSAt 24 hour after paclitaxel (100 nmol/L) treatment, MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map. Six of them were identified by mass spectrometry. They were enolase 1, chloride intracellular channel 1, keratin 8, ribosomal protein S12, galectin-1 and histidine triad nucleotide binding protein, respectively.
CONCLUSIONWe effectively found the changed proteins in the process of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques. These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.
