Changes of biological clock protein in neonatal rats with hypoxic-ischemic brain damage.
- Author:
Yong-Fu LI
1
;
Mei-Fang JIN
;
Bin SUN
;
Xing FENG
Author Information
- Publication Type:Journal Article
- MeSH: ARNTL Transcription Factors; analysis; physiology; Animals; Animals, Newborn; CLOCK Proteins; analysis; physiology; Chronobiology Disorders; etiology; Female; Hypoxia-Ischemia, Brain; metabolism; Male; Pineal Gland; chemistry; Rats; Rats, Sprague-Dawley; Time Factors
- From: Chinese Journal of Contemporary Pediatrics 2013;15(1):62-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of biological clock protein on circadian disorders in hypoxic-ischemic brain damage (HIBD) by examining levels of CLOCK and BMAL1 proteins in the pineal gland of neonatal rats.
METHODSSeventy-two 7-day-old Sprague-Dawley (SD) rats were randomly divided into sham-operated and HIBD groups. HIBD model was prepared according to the modified Levine method. Western blot analysis was used to measure the levels of CLOCK and BMAL1 in the pineal gland at 0, 2, 12, 24, 36 and 48 hours after operation.
RESULTSBoth CLOCK and BMAL levels in the pineal gland increased significantly 48 hours after HIBD compared with the sham-operated group (P<0.05). There were no significant differences in levels of CLOCK and BMAL proteins between the two groups at 0, 2, 12, 24 and 36 hours after operation (P>0.05).
CONCLUSIONSLevels of CLOCK and BMAL1 proteins in the pineal gland of rats increase significantly 48 hours after HIBD, suggesting that both CLOCK and BMAL1 may be involved the regulatory mechanism of circadian disorders in rats with HIBD.
