Methylmalonic acid in amniotic fluid and maternal urine as a marker for neural tube defects.
- Author:
Xiaoping LUO
1
;
Lian ZHANG
;
Hong WEI
;
Wanjun LIU
;
Muti WANG
;
Qin NING
Author Information
1. Department of Pediatrics, Tongji Hospital, Center for the Diagnosis of Genetic Metabolic Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Amniotic Fluid;
chemistry;
Biomarkers;
analysis;
urine;
Female;
Folic Acid;
blood;
Humans;
Methylmalonic Acid;
analysis;
urine;
Neural Tube Defects;
diagnosis;
metabolism;
Pregnancy;
Pregnancy Trimester, Second;
Prenatal Diagnosis;
Vitamin B 12;
blood
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(2):166-169
- CountryChina
- Language:English
-
Abstract:
To evaluate the implication of methymalonic acid (MMA) in the early diagnosis of neural tube defects (NTD), a quantitative assay for MMA was established by using gas chromatography-mass spectrometry with stable isotope of MMA as an internal standard. Amniotic fluid and maternal urine MMA concentration, maternal serum folate, red blood cell folate and vitamin B12 levels were measured in the middle term of NTD-affected and normal pregnancies. Amniotic fluid and maternal urine MMA concentrations in the middle term of NTD affected pregnancies (1.4 +/- 0.9 micromol/L, and 22.1 +/- 12.6 nmol/micromol creatinine) were significantly higher than that of normal pregnancies (1.0 +/- 0. 4 micromol/L, and 2.5 +/- 1.1 nmol/micromol creatinine). There was no significant difference between normal and NTD pregnancies for serum folate, red blood cell folate and vitamin B12 levels. The results suggested that MMAs in amniotic fluid and maternal urine are sensitive markers for early diagnosis of NTD. Vitamin B12 is an active cofactor involved in the remethylation of homocycteine and its deficiency is an independent risk factor for NTD. MMA is a specific and sensitive marker for intracellular vitamin B12 deficiency. This study suggests that it is necessary to monitor the vitamin B12 deficiency and advocates vitamin B12 supplementation with folate prevention program.
