The role of neutrophil collagenase in endotoxic acute lung injury.
- Author:
Tao XU
1
;
Bangxiong ZENG
;
Xingwang LI
Author Information
1. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Endotoxins;
Female;
Lung;
pathology;
Male;
Matrix Metalloproteinase 8;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Respiratory Distress Syndrome, Adult;
chemically induced;
enzymology;
etiology;
pathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(2):196-198
- CountryChina
- Language:English
-
Abstract:
The aim of this study was to determine the role of neutrophil collagenase in the pathogenesis of acute lung injury induced by endotoxin. 28 Sprague-Dawley were randomized into control group and LPS-enduced groups. Samples of left lung were obtained in 2 h (group L1), 6 h (group L2), 12 h (group L3) after intravenous LPS. Immunohistochemsitry was employed for detection of expression of neutrophil collagenase. Pathological scores, lung wet/dry weight ratio and the number of neutrophils were measured. The results showed that the concentration of neutrophil collagenase in LPS-enduced groups (group L1, L2, L3) were significantly higher than that of control group (P<0.01). Pathological scores, lung wet/dry weight ratio and the number of neutrophils in LPS-enduced groups (group L1, L2, L3) were also significantly higher than that of control group (P<0.01). Moreover, among group L1, L2 and L3, there were significant correlations in concentration of neutrophil collagenase and pathological scores, lung wet/dry weight ratio, the number of neutrophils (P<0.05). The present study showed that neutrophil collagenase play an important role in the pathogenesis and progress of endotoxic acute lung injury.
