Cloning of integral mature peptide gene of human GDF-5.
- Author:
Wanshan WANG
1
;
Weiwang GU
;
Qiwei WANG
;
Zhongxian PIAO
;
Yingjie PIAO
Author Information
1. Department of Anatomy, First Military Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- MeSH:
Bone Morphogenetic Proteins;
biosynthesis;
genetics;
Cartilage;
chemistry;
Cloning, Molecular;
Fetus;
Genetic Vectors;
Growth Differentiation Factor 5;
Humans;
Transforming Growth Factor beta;
biosynthesis;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):212-213
- CountryChina
- Language:English
-
Abstract:
The integral mature peptide gene of human growth differentiation factor-5 (GDF-5) was cloned to provide the essential foundation for study on the biological characteristics of GDF-5 at gene and protein levels. Two primers were chemosynthesized according to the hGDF-5 sequence reported in Genbank. The hGDF-5 gene was gained by RT-PCR methods from the total RNA extracted from human fetus cartilage tissue, and was cloned into vector pMD18-T. The sequence of recombinant plasmid pMD18-T-hGDF-5 was analyzed by sequence analysis. DNA agarose gel electrophoresis showed that the product of RT-PCR was about 380bp, and double enzyme digestion of the recombinant plasmid corresponded with it. The result of sequence assay was in agreement with the reported hGDF-5 sequence in Genbank. Our results showed that the integral mature peptide gene of human GDF-5 was cloned successfully from human fetal cartilage tissue, and totally identified with the sequence of human GDF-5 in Genbank.
