Experimental study on the PAR-1 expression around hemotoma following intracerebral hemorrhage in rats.
- Author:
Jingxia GUAN
1
;
Shenggang SUN
;
Xuebing CAO
;
Zhibin CHEN
Author Information
1. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cerebral Hemorrhage;
metabolism;
Female;
Fibrinolytic Agents;
pharmacology;
Hematoma;
metabolism;
Hirudins;
pharmacology;
Male;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Receptor, PAR-1;
biosynthesis;
genetics;
Thrombin;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):266-268
- CountryChina
- Language:English
-
Abstract:
In order to explore the PAR-1 mRNA and protein expression around hemotoma following intracerebral hemorrhage and the relation between the PAR-1 expression and thrombin, collagenase VII was stereotaxically injected into right caudate nucleus in rats. The PAR-1 mRNA expression was detected by RT-PCR method and the PAR-1 protein expression by immunohistochemical method respectively. It was found that the PAR-1 mRNA and protein expression around hemotoma was increased at 6 h after intracerebral hemorrhage (P<0.05), peaked at 2 days (P<0.01), and then declined. The change pattern of the PAR-1 mRNA and protein expression was similar to that of intracerebral hemorrhage after thrombin intracerebral injection. The PAR-1 mRNA and protein expression in hirudin group showed no significant difference with control group. These results indicated that the PAR-1 mRNA and protein expression were markedly increased after intracerebral hemorrhage, which may be closely related to thrombin. Upregulation of the PAR-1 expression may involve in neurotoxic injury induced by thrombin.
