Biocompatibility studies on fibrin glue cultured with bone marrow mesenchymal stem cells in vitro.
- Author:
Huang FANG
1
;
Songlin PENG
;
Anmin CHEN
;
Fengfeng LI
;
Kai REN
;
Ning HU
Author Information
1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biocompatible Materials;
pharmacology;
Bone Marrow Cells;
cytology;
Cells, Cultured;
Coculture Techniques;
Fibrin Tissue Adhesive;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
Rabbits;
Tissue Engineering
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):272-274
- CountryChina
- Language:English
-
Abstract:
By culturing bone marrow mesenchymal stem cells of rabbits with fibrin glue in vitro, the biocompatibility of fibrin glue was investigated to study whether this material can be used as scaffolds in bone tissue engineering. After 2-months old New Zealand rabbits had been anesthetized, about 4-6 ml of bone marrow were aspirated from rabbit femoral trochanter. The monocytes suspension was aspirated after bone marrow was centrifuged with lymphocyte separating medium and cultured primarily. Then the cells were divided into two groups: one was cultured with complete medium and the other with induced medium. The cells of the two groups were collected and inoculated to the culture plate containing fibrin glue. In the control group, cells were inoculated without fibrin glue. The implanted cells and materials were observed at different stages under a phase-contrast microscope and scanning electron microscope. MTT and alkaline phosphatase (ALP) were measured. Bone marrow mesenchymal stem cells grew on the surface of fibrin glue and adhered to it gradually. Cells light absorption value (A value) and the ALP content showed no significant difference. Fibrin glue had no inhibitory effect on cell morphology, growth, proliferation and differentiation. It has good biocompatibility and can be used as scaffold materials for bone marrow mesenchymal stem cells in bone tissue engineering.
