Expression of transforming growth factor-beta in cultured normal human lens epithelia cells.
- Author:
Yukan HUANG
1
;
Houren WEI
Author Information
1. Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. huangyk@public.wh.hb.cn
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Epithelial Cells;
cytology;
metabolism;
Humans;
Lens, Crystalline;
cytology;
metabolism;
RNA, Messenger;
biosynthesis;
genetics;
Transforming Growth Factor beta;
biosynthesis;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):289-291
- CountryChina
- Language:English
-
Abstract:
In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.
