Experimental study on the expression of HIF-1alpha and its relationship to apoptosis in tissues around cerebral bleeding loci.
- Author:
Suiqiang ZHU
1
;
Zhouping TANG
;
Shougang GUO
;
Lan PENG
;
Siyu FANG
;
Suming ZHANG
Author Information
1. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Apoptosis;
physiology;
Brain;
metabolism;
pathology;
Cerebral Hemorrhage;
metabolism;
pathology;
DNA-Binding Proteins;
biosynthesis;
genetics;
Female;
Humans;
Hypoxia-Inducible Factor 1;
Hypoxia-Inducible Factor 1, alpha Subunit;
Immunohistochemistry;
Male;
Middle Aged;
Neurons;
pathology;
Nuclear Proteins;
biosynthesis;
genetics;
Time Factors;
Transcription Factors;
biosynthesis;
genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(4):373-375
- CountryChina
- Language:English
-
Abstract:
The expression of hypoxia inducible factor-1 alpha (HIF-1alpha) and its relationship to apoptosis in tissues around cerebral bleeding loci was studied. The expression of HIF-1alpha and apoptosis in 37 samples of tissues around cerebral bleeding loci and 9 samples of normal cerebral tissues was assessed by immunohistochemical straining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling methods. In 37 tissue samples around cerebral bleeding loci, the positive rate of the HIF-1alpha expression was 40.6%. Especially in the patients with amount of bleeding >60 ml, the positive rate (88.9%) of the HIF-1alpha expression was significantly higher than those with the amount of bleeding ranging from 30-45 ml or 45-60 ml (P<0.05). The expression of HIF-1alpha was increased as the amount of bleeding and operative time increased (P<0.05). There existed a positive correlation between HIF-1alpha labeling index and apoptosis index (n=12, r=0.56, P<0.01). These results suggested that the expression of HIF-1alpha was closely related with the time of hemorrhage and the amount of bleeding, and could induce the apoptosis of neurons.
