Protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes.
- Author:
Nian LIU
1
;
Ronghui YU
;
Yanfei RUAN
;
Qiang ZHOU
;
Jun PU
;
Yang LI
Author Information
1. Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
Adrenergic beta-Antagonists;
pharmacology;
Animals;
Calcium Channels, L-Type;
metabolism;
Carbazoles;
pharmacology;
Female;
Free Radicals;
adverse effects;
Guinea Pigs;
Heart Ventricles;
cytology;
Male;
Myocytes, Cardiac;
metabolism;
pathology;
Oxidative Stress;
Patch-Clamp Techniques;
Propanolamines;
pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(5):433-436
- CountryChina
- Language:English
-
Abstract:
The protective effect of carvedilol on abnormality of L-type calcium current induced by oxygen free radical in single guinea pig ventricular myocytes was studied. Whole-cell patch clamp technique was used to study the effect of H2O2 (0.5 mmol/L) on L-type calcium current in single guinea pig ventricular myocytes and the action of pretreatment with carvedilol (0.5 micromol/L). 0.5 micromol/L carvedilol had no significant effect on ICa,L and its channel dynamics. In the presence of 0.5 mmol/L H2O2, peak current of ICa,L was reduced significantly (P<0.001), the I-V curve of ICa,L was shifted upward, steady-state activation curve and steady-state deactivation curve of ICa,L were shifted left and recovery time of ICa,L was delayed significantly (P<0.001). 0.5 micromol/L carvedilol significantly alleviated the inhibitory effect of H2O2 on ICa,L as compared with that in H2O2 group (P<0.01). In addition, carvedilol reversed the changes of dynamics of ICa,L induced by H2O2. It was concluded that carvedilol could alleviate the abnormality of L-type calcium current induced by oxygen free radical in cardiomyocytes. It shows partly the possible mechanism of the special availability of carvedilol in chronic heart failure.
