Autologous mesenchymal stem cell implantation promotes myocardial expressions of growth factors and improves cardiac function in failing rat hearts
10.3760/cma.j.issn.0253-3758.2009.06.004
- VernacularTitle:自体间充质干细胞植入促进大鼠衰竭心肌生长因子表达、改善心室重构和心功能
- Author:
Ze-Wei TAO
1
;
Long-Gui LI
;
Zhao-Hua GENG
;
Ming-Bao SONG
;
Jia-Rong ZHENG
;
Shi-Yong YU
;
Tao DANG
;
Hua-Li KANG
;
Shan-Jun ZHU
Author Information
1. 第三军医大学附属新桥医院
- Keywords:
Stem cells;
Myocardium;
Ventricular remodeling
- From:
Chinese Journal of Cardiology
2009;37(6):495-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the underlying mechanism of mensenchymal stem cells(MSCs) transfer induced cardiac function improvement in failing hearts.Methods Congestive heart failure(CHF) was induced in rats by cauterization of the heart wall.MSCs were cultured from autologous bone marrow and injected into the border zone and the remote myocardium 5 days after cauterization.Results Ten weeks later,cardiomyocyte nucleus mitotic index,capillary density and expression of insulin-like growth factor 1 (IGF-1),hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) were significantly increased in the border zone and significantly reduced in the remote myocardium in CHF rats (all P＜0.05 vs.sham).Besides cardiac function improvement and left ventricular remodeling attenuation evidenced by hemodynamic and echocardiographic examinations,expressions of IGF-1,HGF and VEGF in the remote myocardium and in the border zone were also significantly upregulated (P＜0.05 or P＜0.01 vs.CHF),and cardiomyocyte nucleus mitotic index as well as capillary density were significantly increased in CHF rats with MSCs(P＜0.05 or P＜0.01 vs.CHF).Moreover,collagen area was significantly reduced and myocardial area was significantly increased in the border zone in these rats too.Conclusion Autologous MSC implantation upregulated expressions of growth factors enhanced cardioangiogenesis which might be the underlying mechanisms for improved cardiac function and attenuated left ventricular remodeling induced by MSCs transplantation in failing rat myocardium.
