Atrial myocytes KChIP2 mRNA expression in rheumatic heart disease patients with atrial fibrillation.
- Author:
Xiao-qiu TAN
1
;
Yan YANG
;
Zhi-fei LIU
;
Zhi-ru BAI
;
Wen ZHOU
;
Jie PEI
;
Gui-lan CHEN
;
Xiao-rong ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Atrial Fibrillation; genetics; Down-Regulation; Female; Humans; Kv Channel-Interacting Proteins; genetics; Male; Middle Aged; Myocytes, Cardiac; metabolism; RNA, Messenger; genetics; Rheumatic Heart Disease; genetics; Shal Potassium Channels; genetics
- From: Chinese Journal of Cardiology 2009;37(6):509-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the KChIP2 mRNA level in rheumatic heart disease patients with or without atrial fibrillation (AF) by real-time PCR.
METHODSRight atrial appendage samples from rheumatic heart disease patients with (n = 17) or without AF (n = 13) were obtained during cardiac surgery. Total RNA was extracted from the atrial tissues, and the KChIP2 and Kv4.3 mRNA were detected by SYBR Green I real-time PCR with the GAPDH as the house keeping gene.
RESULTThe ratio of KChIP2/GAPDH (0.1468 +/- 0.0452 vs. 0.2200 +/- 0.0388, P<0.01) and the ratio of Kv4.3/GAPDH (0.3946 +/- 0.1826 vs. 0.5257 +/- 0.1427, P<0.05) were significantly lower in AF patients compared to non-AF patients.
CONCLUSIONDown-regulated atrial KChIP2 and Kv4.3 mRNA expressions in rheumatic heart disease patients with chronic AF might be one of the molecular bases responsible for the down-regulation of the I(to) current density of AF.
