Atrial myocytes KChIP2 mRNA expression in rheumatic heart disease patients with atrial fibrillation
10.3760/cma.j.issn.0253-3758.2009.06.007
- VernacularTitle:慢性心房颤动患者心房肌KChIP2基因mRNA水平的定量检测
- Author:
Xiao-Qiu TAN
1
;
Yan YANG
;
Zhi-Fei LIU
;
Zhi-Ru BAI
;
Wen ZHOU
;
Jie PEI
;
Gui-Lan CHEN
;
Xiao-Rong ZENG
Author Information
1. 泸州医学院
- Keywords:
Atrial fibrillation;
Potassium channels;
Gene expression
- From:
Chinese Journal of Cardiology
2009;37(6):509-513
- CountryChina
- Language:Chinese
-
Abstract:
Objectlve To detect the KChIP2 mRNA level in rheumatic heart disease patients with or without atrial fibrillation (AF) by real-time PCR.Methods Right atrial appendage samples from rheumatic heart disease patients with (n=17) or without AF (n=13) were obtained during cardiac surgery.Total RNA was extracted from the atrial tissues.and the KChIP2 and Kv4.3 mRNA were detected by SYBR Green I real-time PCR with the GAPDH as the house keeping gene.Result The ratio of KChIP2/GAPDH(0.1468 ±0.0452 VS.0.2200±0.0388,P＜0.01)and the ratio of Kv4.3/GAPDH(0.3946±0.1826 vs.0.5257±0.1427.P＜0.05)were significantly lower in AF patients compared to non-AF patients.Conclusion Down-regulated atrial KChIP2 and Kv4.3 mRNA expressions in rheumatic heart disease patients with chronic AF might be one of the molecular bases responsible for the down-regulation of the Ito current density of AF.
