Microelectrode arrays mapped tissue field action potential duration changes of thoracic spinal cord 1-5 nerves and heart in chronic stress rat model
10.3760/cma.j.issn.0253-3758.2009.06.013
- VernacularTitle:微电极阵列芯片技术在抑郁大鼠胸1～5脊髓与心脏组织场电位变化的应用
- Author:
Yue-Mei HOU
1
;
Zheng-Jiang HU
;
Xiao-Jie MA
;
Juan SUN
;
Yan FENG
Author Information
1. 新疆医科大学附属第一医院
- Keywords:
Adjustment disorders;
Arrhythmia;
Microelectrodes
- From:
Chinese Journal of Cardiology
2009;37(6):532-536
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate chwmc stress induced tissue action potential and pathological changes of thoracic spinal cord 1-5 nerves and heart in SD rats.Methods SD rats(weighing 180-250 g)were randomly divided into depressive group and control group(n=10 each).Depressive model (unpredicted chronic mild stress)waB established according to Gronli's protocol.The heart rhythm,tissue field action potential duration(FAPD)of thoracic spinal cord 1-5 nerves,atrium and veutricle were mapped by microclectrode arrays(MEA)technique.Hcart was sectioned and stained with Massion and HE for pathologicM analysis.Results After 3 weeks chronic stress,P wave[(35.09±7.92)ms vs.(25.43±3.38)ms,P＜0.05]and Q-T interval[(114.64±35.08)ms vs.(81.93±16.35)ms,P＜0.01]were significantly increased,FAPD of thoracic spinal cord 1-5 nerves and heart was significantly prolonged,atrial field action potential duration dispersion(FAPDd)was significantly increased,atrial premature beats(n=2)and ventricular premature beats(n=3)were also recorded in rats from depressive group.Moreover,increased collagen deposition was evidenced in Massion stained myocardium and increased inflammatory cell infiltration in the heart was found by both HE stain and electron microscope from depressive rats.Conclusion Chronic mild stress could activate sympathetic nerves system.promote inflammatory cell myocardial infiltration and myocardial fibrosis,induce arrhythmias by prolonging FAPD and increasing FADPd in thoracic spinal cord 1-5 nerves and/or heart tissue.
