Arteriogenesis induced by intramyocardial recombinant adeno-associated virus vector encoding human CD151 cDNA gene transfer in swines with coronary artery occlusion.
- Author:
Hou-juan ZUO
1
;
Zheng-xiang LIU
;
Xiao-chun LIU
;
He-song ZENG
;
Sha WEN
;
Tao LIU
;
Dao-wen WANG
;
Xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD; genetics; Coronary Occlusion; therapy; Dependovirus; genetics; Female; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Humans; Male; Myocardial Infarction; therapy; Neovascularization, Physiologic; Swine; Swine, Miniature; Tetraspanin 24; Treatment Outcome
- From: Chinese Journal of Cardiology 2009;37(6):537-541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the efficacy of CD151 gene delivery in promoting blood perfusion in swines after myocardial infarction.
METHODSSwines received coronary artery ligation and intramyocardial injection with rAAV-CD151, rAAV-anti-CD151 or rAAV-GFP. Eight weeks after vector injection, Western blot, immunostaining and 13N-labeled NH3 PET were performed to detect gene expression and biological effects of various treatments.
RESULTSHigh level of CD151 protein expression was detected in the rAAV-CD151 group. The capillary density in the rAAV-CD151 group [(83.8 +/- 6.7) n/mm2] was significantly higher than that in the control group [(33.2 +/- 4.5) n/mm2] and rAAV-GFP group [(41.6 +/- 5.6) n/mm2] (all P<0.05); the arteriole density in the rAAV-CD151 group [(16.4 +/- 2.5) n/mm2] was also higher than that in the control group [(6.6 +/- 2.3) n/mm2] and the rAAV-GFP group [(8.4 +/- 1.6) n/mm2] (all P<0.05). However, the lowest capillary density and arteriole density were evidenced in rAAV-anti-CD151 group. Myocardial blood perfusion was significantly increased in rAAV-CD151 group and significantly reduced in rAAV-anti-CD151 group (all P<0.05 vs. control).
CONCLUSIONIntramyocardial injection of rAAV-CD151 could enhance the myocardial express of CD151 protein, increase capillary and arteriole densities and improve blood perfusion in swine with myocardial infarction.
