Arteriogenesis induced by intramyocardial recombinant adeno-associated virus vector encoding human CD151 cDNA gene transfer in swines with coronary artery occlusion
10.3760/cma.j.issn.0253-3758.2009.06.014
- VernacularTitle:重组腺相关病毒介导CD151转染心肌梗死小型猪促进心肌功能性血管形成
- Author:
Hou-Juan ZUO
1
;
Zheng-Xiang LIU
;
Xiao-Chun LIU
;
He-Song ZENG
;
Sha WEN
;
Tao LIU
;
Dao-Wen WANG
;
Xin ZHANG
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Myocardial infarction;
Neovascularization,physiologic;
adeno-associated virus
- From:
Chinese Journal of Cardiology
2009;37(6):537-541
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of CD151 gene delivery in promoting blood perfusion in swines after myocardial infarction.Metheds Swines received coronary artery ligation and intramyocardial iniection with rAAV-CD151,rAAV-anti-CD151 or rAAV-GFP.Eight weeks after vector injection,Western blot,immunostaining and 13N-labeled NH3 PET were performed to detect gene expression and biological effects of various treatments.Results High level of CD151 protein expression was detected in the rAAV-CD151 group.The capillary density in the rAAV-CD151 group[(83.8±6.7)n/mm2]was significantly higher than that in the control group[(33.2±4.5)n/mm2]and rAAV-GFP group[(41.6±5.6)n/mm2](all P＜0.05);the arteriole density in the rAAV-CD151 group[(16.4±2.5)n/mm2]was also higher than that in the control group[(6.6±2.3)n/mm2]and the rAAV-GFP group[(8.4±1.6)n/mm2](all P＜0.05).However,the lowest capillary density and arteriole density were evldanced in rAAV-anti-CD151 group.Myocardial blood perfusion was significantly increased in rAAV-CD151 group and significantly reduced in rAAV-anti-CD151 group(a11 P＜0.05 vs.control).Conclusion Intramyocardial injection of rAAV-CD151 could enhance the myoeardial express of CD151 protein,increase capillary and arteriole densities and improve blood perfusion in swine with myoeardial infarction.
