Effect of adenovirus-mediated tissue factor pathway inhibitor gene transfer on apoptosis of rat vascular smooth muscle cells
10.3760/cma.j.issn.0253-3758.2009.06.015
- VernacularTitle:组织因子途径抑制物基因转移对大鼠血管平滑肌细胞凋亡的影响
- Author:
Yu FU
1
;
Gai-Gai ZHANG
;
Ying CAO
;
Yue LIU
;
Jin-Feng YU
;
Xin-Hua YIN
Author Information
1. 哈尔滨医科大学附属第二医院
- Keywords:
Coronary restenosis;
Gene transfer techniques;
Myocytes,smooth muscle;
Apoptosis;
Tissue factor pathway inhibitorDOI:10.3760/cma.j.issn.0253-3758.2009.06.015
- From:
Chinese Journal of Cardiology
2009;37(6):542-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective Previous studies showed potential role of tissue factor pathway inhibitor (TFPI)on attenuating restenosis,we investigated the effect of TFPI gene transfer on vascular smooth muscle cells(VSMCs)apoptosis.Methods Human TFPI recombinant adenovims or LacZ recombinant adenovirus or PBS were transferred t0 rat aortic VSMCs respectively in vitro.RT-PCR was used to detect the expression of exogenous TFPI gene.VSMCs were examined by cell counting and MTT.Apoptosis of VSMCs was detected by flow cytometry.TUNEL and electron microscope at difierent time after gene transfeL Results mRNA expression of TFPI was detected in VSMCs at the 3rd day after gene transfer.Cell numbers and absorbance value in Ad-TFPI group were similar as those in Ad-LacZ and PBS groups at the 1st,3rd and 5th day but significantly lower at the 7th day(P＜0.05)after gene transfer.The apoptosis rates in Ad-TFPI group tested by flow eytometry were all significant higher than those in Ad-lacZ groups at each time point.The positive rates in Ad-TFPI group determined by TUNEL were significant highcr than those in Ad-LacZ groups at 3rd (10.82%±1.57%vs.3.46%±0.93%).5th and 7th(16.95%±2.01%vs.5.11%±1.29%,all P＜0.05)day post gene transfer.Electron microscope evidenced cell contracting,cytoplasm condensing,lighfly swelled mitochondria.nucleus pyknosis and apoptotic body formation after gene transfer in Ad-TFPI group which were not shown in cells of LacZ and PBS groups.Conclusion TFPI gene transfer could induce apoptosis in rat VSMCs which might be one of the mechanisms responsible for its beneficial effect on restenosis inhibition after angioplasty.
