Kv1.3 potassium channel expression changes after CD4+ and subsets CD28null/CD28+ T cells activation in peripheral blood of patients with acute coronary syndrome
10.3760/cma.j.issn.0253-3758.2009.07.008
- VernacularTitle:急性冠状动脉综合征患者外周血CD4+T细胞及CD28null/CD28+亚型活化后Kv1.3钾通道的表达和意义
- Author:
Da-Ying FENG
1
;
Cun-Tai ZHANG
;
Ye-Xin MA
;
Hong-Lian ZHOU
;
Ren-De XU
;
Xin-Wei YANG
;
Shen HUANG
;
Jin MA
;
Xiao-Qing QUAN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Coronary artery disease;
T lymphocyte;
Potassium channel
- From:
Chinese Journal of Cardiology
2009;37(7):599-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the Kv1.3 channel expression changes after CD4 + and subsets CD28null/CD28+T cells activation in peripheral blood of patients with acute coronary syndrome (ACS).Methods CD4+ T cell in 27 ACS patients and CD4+ CD28null/CD4+ CD28 + T cells in 12 out of these 27 ACS patients were isolated from peripheral blood with magnetic cell sorting.The whole-cell Kv1.3 currents for three T cells were recorded with patch-clamp technique before and 72 hours after activation by purified anti-human CD3 Interferon gamma,tumor necrosis factor alpha(TNF-α),granzyme B mRNA expression were determined by reverse transcription-PCR before and 72 hours after activation by purified anti-human CD3 in the presence or absence of recombinant Margstoxin (rMgTX,0.1,1,10 nmol/L),a specific Kv1.3 channel blocker.Results Peak Kv1.3 channel currents of CD4 + ,CD4+ CD28null,CD4+ CD28 + T cells were significantly increased and the mean Kv1.3 channel numbers per cell of these cells were increased by about 90%,60%,80% (402 ± 88 vs.752 ± 275,553 ± 328 vs.874 ± 400,392 ± 133 vs.716 ± 251,all P <0.05) after activation compared to baseline values.Baseline CD4+ CD28nullT cell numbers were about 40% more than those of CD4+ CD28+ T cell (P < 0.05) and were similar after activation (P = 0.102).The mRNA expression of interferon gamma,TNF-α and granzyme B were dose-dependently down-regulated by rMgTX.Conclusions Kv1.3 channels of peripheral CD4 + T cell and CD28null/CD28 + T cells from ACS patients significantly increased after activation and Kv1.3-specific channel blocker rMgTX could effectively abolish this effect suggesting a potential role of Kv1.3 channel blocker on plaque stabilization in ACS patients.
