Overexpression of angiotensin converting enzyme 2 inhibits inflammatory response of atherosclerotic plaques in hypercholesterolemic rabbits
10.3760/cma.j.issn.0253-3758.2009.07.014
- VernacularTitle:血管紧张素转化酶2基因转染对实验性动脉硬化兔斑块炎症的影响
- Author:
Bo DONG
1
;
Yue-Hui ZHANG
;
Qiu-Li DONG
;
Qing-Tao YU
;
Li ZHU
;
Shu-Ying LI
;
Ya-Pei YANG
;
Cheng ZHANG
;
Jin-Bo FENG
;
Chun-Xi LIU
;
Huai-Dong SONG
;
Chun-Ming PAN
;
Yun ZHANG
Author Information
1. 山东大学附属省立医院
- Keywords:
Atherosclerosis;
Peptidyl-dipeptidase A;
Gene;
Transfection
- From:
Chinese Journal of Cardiology
2009;37(7):622-625
- CountryChina
- Language:Chinese
-
Abstract:
Objective Angiotensin converting enzyme 2 (ACE2.) efficiently hydroiyses the potent vasoconstrictor angiotensin Ⅱ to vasodilative angiotensin (1-7).We hypothesized that ACE2 overexpression may inhibit inflammation response in atherosclerotic plaque by degrading Ang Ⅱ into Ang-(1-7).Methods Atherosclerosis (AS) plaques were induced in the abdominal aorta of 38 rabbits by endothelial injury and atherogenic diet for 3 months.Rabbits were then underwent injection of a recombinant adenovirus (2.5 × 109 pfu/ml) carrying a murine ACE2 gene (Ad-ACE2) through a catheter into the abdominal aortic segments rich in plaques (n = 19) or injection of a control vector Ad-EGFP (n = 19).One month later,all rabbits were sacrificed and plaques from aortic segments were analyzed.Results ACE2 expression in aortic tissues of the Ad-ACE2 group were confirmed by immunohistochemistry.Mcrophage infiltration (13.6% ± 4.2% vs.23.6% ±6.9%,P<0.01) and MCP-1 expression (13.2%±0.4% vs.25.0%±7.4%,P<0.01) were significantly reduced in Ad-ACE2 group compared to Ad-EGFP group.Conclusions Overexpression of ACE2 inhibited atherosclerotic plaque inflammation response in hypercholesterolemic rabbits.
